Variations in the … Brown fat is important for thermoregulation in newborns and hibernating mammals. White fat cells or adipocytes are specialized for the storage of triglyceride, and occur singly or in small groups scattered throughout the loose connective tissue. The basal lamina is anchored to the underlying connective tissue by fine fibrils oftype VII collagen (you obviously can't tell this looking at it in the light microscope, but you should recall this from lecture). 4 Connective tissues Most of the body is made of connective tissues. In this image of an artery, What type of fibers are stained darkly? Elastic fibers contain the protein elastin, which co-polymerizes with the protein fibrillin. As fibroblasts are the most common cells in areolar tissue, the majority of the nuclei seen here are probably fibroblast nuclei. Connective tissue is made up of cells and extracellular matrix. Loose connective tissue is divided into 1) areolar, 2) adipose, 3) reticular. Look at the connective tissue in the submucosa which is the lighter staining area View Image between the intestinal epithelium and the smooth muscle layer. In this section, the fibers clearly predominate. Mast cells are actively involved in a host's immune response and produce many substances, some of which are heparin and histamine. IgE is an antibody, produced by plasma cells, that has a high affinity for mast cells and basophils. You should be able to recognize a range of nuclear morphologies and be able to identify the cells as fibroblasts. You will encounter each of these later in the course; for now, make sure you recognize that they all descend from monocytes, and that the macrophage is the connective tissue version. 7 Types of Connective Tissue - Microscope Slides learn by taking a quiz; Online quiz to learn 7 Types of Connective Tissue - Microscope Slides; Your Skills & Rank. Slide 160 (stomach, PAS & Azure II) View Virtual Slide. Observe the branching nature of the elastic fiber and the "mantle" of elastic microfibrils. The fibroblast synthesizes the collagen and ground substance of the extracellular matrix. Note the alternating layers of fibroblasts and collagenous fiber bundles. The fibrous components of loose areolar connective tissue, when viewed with the light microscope, appear as colourless strands of varying diameter running in all directions, and, if not under tension, these have a slightly undulant course. A collagen fibril -the brackets enclose a single collagen FIBRIL, which is made of 1000s of tropocollagen units attached in a head-to-tail arrangement. Locate and classify the connective tissue in this image of the intestine. Bones and cartilage are the two types of connective tissue in this sub-category. You are not responsible for recognizing reticular fibers unless a silver stain is used. These are cells (or adipocytes). Both are fluid, … Loose Connective Tissue. Slide 106 and 112 have bits of well preserved flexor tendon at the top of the section (the tissue at the very top of slide 112 is actually skeletal muscle -which you'll study in the next lab; the tendon is just below it). Please read the descriptions of the connective tissue stains BEFORE you do your lab work. This tissue section stained with aldehyde fuchsin and eosin. As they have a phagocytic function, removing pathogens and cell debris, macrophages usually contain abundant primary and secondary lysosomes. These cells can grow up to 100 microns and usually contain once centrally located vacuole of lipid - the cytoplasm forms a circular ring around this vacuole, and the nucleus is compressed and displaced to the side. Its high concentration of negatively-charged sulfate groups makes it appear intensely basophilic under H&E. You should also observe that there aren't a lot of cells, a characteristic of "dense" connective tissue. Stain results: Septa: Red: ... Electron Microscopy Sciences specializes in the manufacturing, preparation and distribution of the highest quality laboratory chemicals and microscopy supplies and equipment for electron microscopy, light microscopy, and histology. Finally, recall that PROcollagen is INTRAcellular and doesn't aggregate into fibrils as shown here until secreted. In addition to some fibroblasts and a few delicate collagen fibers, you should see quite a few plasma cells View Image amongst the epithelial tubes. In contrast to epithelia, connective tissue is sparsely populated by cells and contains an extensive extracellular matrix consisting of protein fibers, glycoproteins, and proteoglycans. Slide 88 is stained with H&E where the concentric rings of elastic lamellae are intensely stained with eosin giving a glassy red appearance View Image (one of the few places where elastin is easy to recognize in H&E sections). Dense connective tissue is full of fibroblasts. The luminal surface of the intestine is made up of a simple columnar epithelium. Be sure you look at both the H&E and Masson trichrome-stained slides as they provide an excellent opportunity to see how collagen stains in connective tissue when either stain is applied. Non-striated (involuntary) muscle E 5 Striated muscle, showing striations and nuclei LS 5 Adipose tissue, section 5 Pseudo-stratified ciliated: epithelium TS 5 Areolar tissue, stained for fibres and cells E 5 Spinal cord TS 5 Compact bone, for Haversian Canals and lamellae 5 Cardiac muscle showing the nuclei, striations and intercalated discs LS 5 None of our slides of adult tissue shows any brown fat, however this rather unique tissue can be seen in slide H2 View Image, which is from a developing fetus. Macrophages are the biggest, rounded cells that are floating free in the spaces between the cords of cells. Histological for human physiology. The very small cracks between the fibers are just artifacts of shrinkage that occured during tissue preparation. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Connective tissue - blood (Note: Erythrocytes - red blood cells; have hemoglobin; don't have nuclei because they lose it when made from bone marrow. You will study neutrophils in much greater detail in other sequences and in your histopathology course, but it is useful for now to at least be able to recognize them in various tissues and organs. What functions does this type of connective tissue play to support the epithelium? The antibody binds mast cells and waits for a second exposure to whatever it happens to be responsive to (an allergen). 2. In these particular instances, don't worry so much about trying to exactly classify the tissue per se, but at least try to identify the cellular and extracelluar components that you can (also bear in mind that you can't always definitely identify every cell) and think how its overall appearance reflects its function. 21 Plasma cell View Virtual EM Slide Areolar Connective Tissue 65-81, Supporting/Connective tissues, Ross and Pawlina (6th ed), Chapter 6 Connective Tissue, pgs. Connective tissue cells are usually divided into two types: Fixed cells (or resident cells) - resident population of cells that develop and remain within connective tissue. This quiz only covers material that one would be expected to discuss in a histology laboratory. They are especially common along smaller blood vessels. Ground substance is an aqueous gel of glycoproteins and proteoglycans that occupies the space between cellular and fibrillar elements of the connective tissue. Location. Muscle tissue is specialized for contraction. This region is also a loose, irregular connective tissue but can be so extensively infiltrated by white blood cells and plasma cells that the supporting fibers and ground substance are obscured. Connective tissue is divided into four main categories: Connective proper; Cartilage; Bone; Blood; Connective tissue proper has two subclasses: loose and dense. Reticular fibers are composed of type III collagen. As you look at this section, you will see a very obvious layer of mucous epithelial cells (PAS also reacts with the carbohydrate-rich mucin). A final, special connective tissue is vascular or blood tissue. Many of these macrophages contain phagocytosed red blood cells or the brownish breakdown pigment, hemosiderin (which is the result of lysosomal action on the ingested red blood cells.). Chapter 3 - Connective Tissue. Note the uniform distribution of regularly arranged collagen fibers (type I). This system consists of a number of tissue-specific, mobile, phagocytic cells that descend from monocytes - these include the Kupffer cells of the liver, the alveolar macrophages of the lung, the microglia of the central nervous system, and the reticular cells of the spleen. Usually there is more extracellular matrix than cellular material. The fine collagenous network that provides support in the bone marrow, lymphatic organs, around individual smooth muscle cells, and beneath most epithelia is composed mainly of Type III collagen. 4. They release histamine during allergic reactions. Slide 26 (lymph node, H&E) View Virtual Slide. What is their function. 1. Neutrophils generally enter tissues in large numbers only in response to a disease stimulus. NOTE: Slide 250 illustrates a point about the limits of classification schemes. Just as in the H&E-stained aorta, the elastic fibers in the pharynx and trachea are glassy and orange-red --they appear as stippled dots because they’ve been cut in cross section. Brown fat cells are highly specialized for temperature regulation. If you are a University of Michigan student enrolled in a histology course at the University of Michigan, please click on the following link and use your Kerberos-password for access to download lecture handouts and the other resources. Dyes or carbon particles ( spindle-shaped ) cells of the intestine INTRAcellular and n't. Also be foundwithin the connective tissues one side the nuclei seen here are probably fibroblast nuclei cells are granulated typically. The luminal surface of the tissue volume in white or unilocular adipose tissue,.! Empty '' looking intercellular space a thin reticular network - dense irregular, wavy fibers... What you can identify a macrophage and not distinguishable ( evenly dark and round ) euchromatin. 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