Now in its fourth edition, Pathology of Laboratory Rodents and Rabbits has become a standard text for veterinary pathologists, laboratory animal veterinarians, students, and others interested in these species. ISBN number: 3-437-30692-8 (Stuttgart) or 1-56081-354-7 (New York). The tray of sterile instruments is lifted out of the sterilizer and laid on a spread towel which has previously been soaked in 1: 1,000 solution of mercuric chloride. For the success of this operation very sharp needles are essential. … This method is mainly used in testing cultures of the diphtheria bacillus for toxigenicity. The health and well-being of the animals depend on the care, human attitude of the animal-keepers (staff) of the animal house. Animal rooms have to be constructed to appropriate standards and containment levels, just as laboratories. After the removal of the organs these are placed in separate Petri dishes. A suitable animal board or table, on the top of which the carcase can be fixed in the supine position should be used. Failing this, the operator should use a mask. The animal is anaesthetized and then fastened to a board with the body axis quite straight and the fur clipped over the left side of the chest; the area is shaved and then sterilised with alcohol and ether. The tail vein is dilated by placing in water at about 45°C. The rabbit is picked up from cage with the ears by one hand in a firm grip and another hand is placed under the hind-quarters to support the weight and then lifted gently. Blood can thus be withdrawn and inoculated into various media. (i) Newly arrived animals to be kept in a special quarantine room and kept under observation for 10-14 days. With a sharp downward movement inclined towards the midline is then made that takes the needle point through the ventricular wall. Before operation, heated (by Bunsen burner) petroleum jelly is kept ready in a container. Diagnostic Laboratory. 20 gauge mounted on a syringe) is inserted at this point between the ribs. Under light ether anesthesia, as soon as the regular respiration is establi­shed, 0.5 to 1.5 ml material is dropped from a Pasteur pipette into the nares. This diagnostic microbiology manual is designed to be used in conjunction with lecture textbook and other resources. Experimental Procedure Inoculation of Material: At first the animal is clipped and its flank is shaved to remove the hair. After the tissue has settled to the bottom of the mortar, the supernatant fluid is drawn into a syringe. Farmers and other food producers use the results of this work to keep animals healthy and meat products safe for human consumption. Instruments are removed from the sterilizer. Data’s of  Laboratory Animals 3. European hedgehog bred in captivity is suitable for propagation. Please find information on our services and visiting restrictions in our COVID-19 section. Cages may be boiled in soapy water; alterna­tively, to be kept immersed in a solution of disinfe­ctant such as 3% Lysol. The use of microbial cultures is common to help in the clinical identification of pathogenic microbes. Metal instruments are sterilised by boiling in a sterilising bath, e.g. Answer Now and help others. Rabbits may be marked in ears with a needle dipped in India ink. Preparation of Microbiology Samples in Animals Any specific agents of interest in the diagnostic investigation should be mentioned on the submission form; some agents have requirements (eg, anaerobic culture, special media) that would not be used in most laboratories unless the pathogen was cited as a differential diagnosis. Only one eye is inoculated by dropping a drop of material through a Pasteur pipette. A wide-bore transfusion needle is fitted into a short length (1 ½ in) of thick rubber tubing and sterilised by boiling. Growths on solid media are first scraped off and suspended in broth or saline, alternatively, the diluting fluid may be poured on the culture which is then emulsified with a wire loop. While there are significant differences in how these have been developed and implemented in each country, they are based on basic ethical principle… Diarrhoea due to Salmonella typhimurium is a common natural disease of rabbits, guinea pigs and mice. When the jelly becomes cool but still fluid, the jelly is painted over the vein — both on margin and underside of the ear. Rubber gloves, instru­ments and tray are thoroughly sterilised. Animal is then fixed to the board and towels moistened with antiseptic solution are placed over the head and lower extre­mities of the animal. A specific section for veterinary diagnostic laboratories addresses the veterinary issues not shared by other human laboratory departments. When tissue is well-ground, more saline is added and allowed to stand for a short time. When the necropsy has been properly performed, it is not necessary to scar the surface of the heart. Animals belong to mammalia class Diagnostic microbiology. Other methods include polymerase chain reaction (PCR) and immunostaining. Method (2) is perhaps easier. Isolation of virus from brain tissue and/or saliva. 100% 100% found this document useful, Mark this document as useful. This website includes study notes, research papers, essays, articles and other allied information submitted by visitors like YOU. This covers a wide field of knowledge comprising veterinary bacteriology, water microbiology and food bacteriology. Vein may be distended by rubbing with a piece of cotton wool or by holding the ear on an electric bulb (heat dilates blood vessels). Care and Management of Laboratory Animals 2. 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