d. It is done by heating or the addition of methanol. The specific name, cereus, meaning waxy in Latin, refers to the appearance of colonies grown on blood agar. Image Based Life > Uncategorized > can bacillus grow on mannitol salt agar You skipped the crystal violet step. Staphylococci other than S. aureus (e.g. c. evaporation of the media d. Chains of rod-shaped bacteria c. It acts as a mordant, increasing the cells' affinity for the stain. d. non-enveloped viruses c. Add decolorizing agent. B. subtilis is found in soil and the gastrointestinal tract of ruminants and humans. True/False a. coagulase . hydrogen peroxide Expert Answers: Micrococcus are aerobic, Gram-positive cocci ranging in size from 0.5 to 2.0 m in diameter. The preferred medium is M9 with glucose. What happens to the Gram-positive cell wall during decolorization? Can bacillus subtilis grow on blood . (enables them to grow). This cookie is set by GDPR Cookie Consent plugin. This cookie is set by GDPR Cookie Consent plugin. Gram-negative bacteria like E. coli and P. aeriginosa are not tolerant to salt (not halophilic) and will not grow colonies on MSA (see quadrants II and IV). What happens to the Gram-negative cell wall during decolorization? This cookie is set by GDPR Cookie Consent plugin. But the genus Staphylococcus has a protective slime layerthat protects it in a harsh, salty environment. c. fibrin Chocolate agar is used for growing fastidious respiratory bacteria, such as Haemophilus influenzae and Neisseria meningitidis. Pancreatic digest of casein, peptic digest of animal tissue, and beef extract are the nutritional . True/False a. What describes a microorganism that can SURVIVE exposure to a high temperature, but may not necessarily GROW at a high temperature? It does not store any personal data. When inoculated on tryptic soy agar plates, both bacterial types show growth. Begin typing your search term above and press enter to search. A basic stain colors the background of the slide; an acidic stain penetrates the cells themselves. Bacillus subtilis is a spore forming, motile, rod-shaped, Gram-positive, facultative aerobe. While this does not initially seem logical, there are reasons behind it. b. Bacterial growth is apparent along the streaks connecting each quadrant. Streptococcus pyogenes is sensitive to growth inhibition by bacitracin, whereas Streptococcus agalactiae (group B streptococci) is not. Mannitol salt agar is a selective medium for isolation, differentiation, and enumeration of Staphylococcus species from clinical and non-clinical samples. c. Bacillus subtilis Is Bacillus subtilis anaerobic or aerobic? Which of the following substances is used as a decolorizing agent in the Gram stain? It also contains the carbohydrate mannitol, and a pH indicator, phenol red, which allow distinction between organisms which can ferment mannitol and those that can not. Bacara is a chromogenic selective and differential agar that promotes the growth and identification of B. cereus, but inhibits the growth of background flora. If the bacteria is able to grow then it is a halophilic bacteria, due to it's ability to grow in a high salt environment. Each person will use one-half of a mannitol salt agar plate. The colonies of Staphylococcus aureus at the top are surrounded by a yellow zone . Which of the following is the best description of mannitol salt agar MSA? The bacteria are commonly found in the environment and can contaminate food. identify S.aureus. d. ferments mannitol When cultured on ordinary nutrient agar, the morphology circular colony of this bacteria is rough, opaque, fuzzy white or slightly yellow with jagged edges . b. Staph. Mannitol Salt Agar (MSA) is used to determine if the bacteria is halophilic (salt loving) and if the bacteria can ferment mannitol. Cultivation Media for Bacteria. Mannitol fermenting organisms like B. megaterium yield yellow coloured colonies as shown in Figure 4. the selective agent. b. It's a site that collects all the most frequently asked questions and answers, so you don't have to spend hours on searching anywhere else. No, enterococci cannot grow on mannitol salt agar because are salt resistant bacteria but they are able to ferment mannitol and produce lactic acid, creating colonies of yellow color on MSA. Mannitol Salt Agar is used as a selective media for the isolation of pathogenic Staphylococci. Mannitol Salt Agar (MSA) is a selective and differential medium. Can Streptococcus grow on mannitol salt agar? If you forget to add the decolorizing agent when performing a Gram stain, what color will the gram-negative cells be? c. Phenol red permits the differentiation of bacteria based on their ability to ferment mannitol. Organisms that ferment lactose will form colorless colonies on MAC. Organisms from other genera may grow, but they typically grow very weakly. Cells retain their natural size and shape 1 Would Bacillus cereus Grow on mannitol agar? The bacterial cells will crenate. Mannitol Salt Agar (MSA) is used to determine if the bacteria is halophilic (salt loving) and if the bacteria can ferment mannitol. Mannitol is not fermented by isolate if growth and surrounding medium are eosin pink. The optimal temperature is 25-35 degrees Celsius (Entrez Genome Project). BACARA agar is a selective chromogenic medium that allows the enumeration of Bacillus of the cereus group without confirmation. Certain agar's however, are specifically enriched for c. Clusters of gram-negative bacilli What specific bacterial species can MSA identify? c. Heat fixation is recommended when staining with an acidic stain, but not when staining with a basic stain. Which category of bacteria do not grow on MSA? b. Rinse the smear with decolorizing agent until the smear has no more color. This cookie is set by GDPR Cookie Consent plugin. Mannitol Salt Agar (MSA) This is a medium that is both selective and differential. I am Kevin Tokoph, PT, DPT. The MSA will select for organisms such as Staphylococcus species which can live in areas of high salt concentration (plate on the left in the picture below). The Serratia marcescens does not grow because of the high salt content. We use cookies to ensure that we give you the best experience on our website. Staphylococcus epidermidis will grow on PEA. It can quickly multiply at room temperature with an abundantly present preformed toxin. Except the last-mentioned, the phenotypic differentiation of species between Bacillus cereus group is impossible with actual culture methods. What kind of bacteria can grow on mannitol salt agar? Optimal growth occurs within the narrower temperature range of 82F (28C) to 95F (35C) and a pH range of 4.9 to 9.3 (FDA 2012b). E coli is a major facultative inhabitant of the large intestine. c. Acidic stains have a positively charged chromogen and are repelled by the positive charges on bacterial surfaces. b. cannot ferment mannitol a. Out of these, the cookies that are categorized as necessary are stored on your browser as they are essential for the working of basic functionalities of the website. Listeria monocytogenes hydrolyses aesculin resulting in the formation of a black halo around colonies. 4 What kind of bacteria can grow on mannitol salt agar? Other uncategorized cookies are those that are being analyzed and have not been classified into a category as yet. What is the selective ingredient in Mannitol Salt Agar? Can a mannitol isolate be fermented by isolate? Pseudomons fluorenscens June 8, 2022; group homes for adults with mental disabilities; 24x48 polished porcelain tile . MAC selects FOR gram negative bacteria. In this experiment, the presence of yellow halos around colonies growing on mannitol salt agar indicates that the microbe ___________. Mannitol Salt Agar is used as a selective media for the isolation of pathogenic Staphylococci. Mannitol salt agar has a pH indicator in the form of phenol red. subtilis is able to complete glycolysis and the TCA (tricarboxylic acid) cycle because of its aerobic cellular respiration. a. The MacConkeys and Mannitol Salt described above are both selective and differential bacterial growth media. This resulted in the growth of bacteria and the MSA test was concluded to be positive for mannitol fermenters. Most non-pathogenic staphylococci will not ferment mannitol. Bacillus subtilis is not able to ferment mannitol and yet the Mannitol test yielded a positive result. Preparation of Mannitol Salt Agar (MSA) Suspend 111 grams of Mannitol Salt Agar in 1000 ml of distilled water. What kind of bacteria can grow on mannitol salt agar? c. Isolated colonies are all white in color, but some colonies are noticeably larger than others. Which Teeth Are Normally Considered Anodontia. Acidic stains have a positively charged chromogen and are repelled by the negative charges on bacterial surfaces. What purpose does phenol red serve in MSA? ___________________ media incorporate chemical substances that inhibit the growth of one type of bacteria while permitting the growth of another. which turns the agar yellow from red. 8 Can a mannitol isolate be fermented by isolate? Most known bacteria that have been cultured from human skin are Gram-positive. The MSA agar will retain its initial red color and will not change to yellow. a. Mannitol salt agar c. Soft soap (Antimicorbial) c. Air-dry. 7 Will Bacillus Grow on mannitol salt agar? 5 What bacteria can grow on Cetrimide Agar? What kind of media is Mannitol Salt Agar? b. Pseudomonas aeruginosa True/False Mannitol Salt Agar (MSA) is a selective and differential medium. a. Bacterial growth is apparent in all four quadrants. Staphylococcus aureus will grow on PEA, Which organism was tolerant of the highest temperatures? You forgot the decolorizing step. What media grows in addition to most nonfastidious bacteria, will also support growth of pathogenic species of the genus Neisseria as well as Haemophilus influnzae? Which of the following is an example of an acidic stain? The cookie is used to store the user consent for the cookies in the category "Analytics". Some group D enterococci may exhibit growth with mannitol fermentation; however, catalase test and gram morphology should distinguish between enterococci and staphylococci. d. separating cells within the solid surface. Welcome to Catalyst University! Bacillus , and some species of Serratia . When the Bacillus subtilis was isolated on the Mannitol Salt Agar plate, the color of the plate also changed from red to yellow. This website uses cookies to improve your experience while you navigate through the website. This is a selective and differential media which grows and differentiates Staphylococcus species. Bacillus subtilis can grow in any medium containing nitrogen and carbon source with Nacl. The high concentration of salt (7.5%) selects for members of the genus Staphylococcus, since they can tolerate high saline levels. While this does not initially seem logical, there are reasons behind it. S.epidermidis will grow, but the agar remains red. Which of the following objective lenses is the ONLY lens that should be used with oil immersion? By clicking Accept All, you consent to the use of ALL the cookies. Mannitol (Mtl) fermentation, with the subsequent production of acid, is a species signature of Staphylococcus aureus, and discriminates it from most other members of the genus. Laboratory 3 02/24/2023 (Tuesday Section; Session #1 2:45-4:40 PM) Objectives Objective 1: To aseptically inoculate a nutrient broth with a broth culture of E. coli. True/False Mannitol Salt Agar contains peptones and beef extract, which supply nitrogen, vitamins, minerals and amino acids essential for growth. How are MSA and Mac both selective and differential? b. human serum Staphylococcus aureus grows on this medium and ferments mannitol to produce yellow colonies. When the Bacillus subtilis was isolated on the Mannitol Salt Agar plate, the color of the plate also changed from red to yellow. b. Grape-like clusters of ball-shaped bacteria a. Cells are not distorted by the effects of heat What is the purpose of adding phenol red to mannitol salt agar? Do you need underlay for laminate flooring on concrete? The pH indicator in MAC is phenol red. When it ferments D-mannitol, it produces an acid which turns the agar yellow from red. You decolorized too much. Organisms capable of using mannitol as a food source will produce acidic byproducts of fermentation that will lower the pH of the media. These cookies ensure basic functionalities and security features of the website, anonymously. Gram negative bacteria grow well on PEA. Staphylococcus aureus and Staphylococcus epidermidis on mannitol salt agar. c. Gamma rays Broth dilution tests provide ________________ results. TimesMojo is a social question-and-answer website where you can get all the answers to your questions. e. Spiral-shaped bacteria, Bacillus anthracis appears as chains of rod-like cells. The growth follows the typical four phases of bacterial growth (figure 2) and with reaching the exponential growth phase, Bacillus subtilis has a doubling time of 30 minutes under ideal conditions. b. Pyrogens act on the hypothalamus of the brain where they cause the release of ________. Add safranin. aureus grow on Mannitol Salt Agar. What would be the likely consequences of omitting the NaCl in Mannitol Salt Agar? It does not store any personal data. In the streak-plate technique, the intent is to isolate bacteria by dilution in theory by __________. d. Phenylethyl Alcohol Agar (PEA). subtilis) is a Gram-positive, aerobic bacterium. Home | About | Contact | Copyright | Report Content | Privacy | Cookie Policy | Terms & Conditions | Sitemap. The many species of the genus exhibit a wide range of physiologic abilities that allow them to live in every natural environment. What bacteria does mannitol salt agar inhibit? a. From the metabolic point of view it has catalase, reduces nitrates to nitrites, gives the Voges Proskauer reaction, ferments glucose, sucrose, salicin and glycerol, does not ferment mannitol, nor arabinose and produces lecithinase. Mannitol Salt Agar (MSA) . What is its moment of inertia about L2L_2L2 ? These cookies track visitors across websites and collect information to provide customized ads. What would be the effect on decolorization? Before receiving an injection, rubbing alcohol is typically applied to the skin to decrease the number of microbes at the injection site. d. None of the above. c. Bacteriophage 1 Can Bacillus megaterium ferment mannitol? "normal" T-Soy (no supplementation) If the color is red, it is the bacteria is basic. When the Bacillus subtilis was isolated on the Mannitol Salt Agar plate, the color of the plate also changed from red to yellow. 2 What bacteria does mannitol salt agar inhibit? The high concentration of salt (7.5%) selects for members of the genus Staphylococcus, since they can tolerate high saline levels. What type of organisms grow in mannitol salt agar? As the name suggests, it contains cetrimide, which is the selective agent against alternate microbial flora. d. An acidic stain has a negatively charged chromogen and a basic stain has a positively charged chromogen. We use cookies on our website to give you the most relevant experience by remembering your preferences and repeat visits. d. The bacterial cells will undergo osmotic lysis. MacConkey agar is a selective and differentiating agar that only grows gram-negative bacterial species; it can further differentiate the gram-negative organisms based on their lactose metabolism. e. Capsule, Four-way quadrant streak plates are useful in microbiology to __________________. Press ESC to cancel. a. If desired, sterile Egg Yolk Emulsion (E7899) can be added to a final concentration of 5% v/v after autoclaving. c. Spirilla Just clear tips and lifehacks for every day. a. Protozoa aureus strains will not ferment mannitol. d. You forgot the Gram's iodine step. This cookie is set by GDPR Cookie Consent plugin. Gram positive bacteria grow well on PEA. Cultural Characteristics of Bacillus subtilis. Analytical cookies are used to understand how visitors interact with the website. 350 nm Staphylococcus species, which commonly inhabit human skin, can grow on this high salt concentration (left plate in picture below). c. Staphylococcus aureus It binds to crystal violet, forming an insoluble complex. c. Gram-negative cocci You performed the Gram stain on a smear. d. Archaea What is the most critical step in the Gram staining procedure? c. Over time, gram-negative cells gain the ability to retain the crystal violet stain after decolorization. The Gram stain is an example of a __________ stain, because the process uses two contrasting stains to separate bacteria into groups based on cell wall composition. What is the lowest concentration of a substance that prevents bacterial growth? But opting out of some of these cookies may affect your browsing experience. Bacillus subtilis is not able to ferment mannitol and yet the Mannitol test yielded a positive result. a. Low-power lens (10X) a. ; In spite of the diversity, the colonies of Bacillus species can be recognized on agar plates quite easily. b. Ethyl alcohol (C2H5OH) It contains a high concentration (about 7.510%) of salt (NaCl) which is inhibitory to most bacteria making MSA selective against most Gram-negative and selective for some Gram-positive bacteria (Staphylococcus, Enterococcus and Micrococcaceae) that tolerate high salt concentrations. Sorbitol MacConkey Agar (SMAC) Bile salts and crystal violet inhibits the growth of most Gram positive, non-enteric bacteria. Why is it recommended that Gram staining be performed on young bacterial cultures (not older than 24 hours)? A dog typically pants at a rate of 670 pants per minute. B. anthracis. It is recommended for the detection and enumeration of coagulase-positive Staphylococci in milk, food and other specimens. 4 What bacteria can grow on mannitol salt agar? cereus. Additionally, mannitol salt agar contains the sugar mannitol. c. Par focal b. Does Bacillus grow on MSA? Mannitol salt agar has 7.5% salt. These data reveal that we now have rapid, cost effective ways to identify enterococci to the species, and not just genus, level and have significance for patient treatment in hospitals. Mannitol Salt is a selective bacterial growth medium because it has a very high concentration of NaCl (7.5%). A negative stain allows you to see structures inside cells. Mannitol Salt Agar is not completely selective for . What kind of bacteria can grow on mannitol? When the Bacillus subtilis was isolated on the Mannitol Salt Agar plate, the color of the plate also changed from red to yellow. a. colorless; purple Do Men Still Wear Button Holes At Weddings? b. c. dilution on a solid surface We use cookies on our website to give you the most relevant experience by remembering your preferences and repeat visits. d. The smear preparation is too light. If the bacteria is able to grow then it is a halophilic bacteria, due to its ability to grow in a high salt environment. b. yellow colonies on pink agar EMMY NOMINATIONS 2022: Outstanding Limited Or Anthology Series, EMMY NOMINATIONS 2022: Outstanding Lead Actress In A Comedy Series, EMMY NOMINATIONS 2022: Outstanding Supporting Actor In A Comedy Series, EMMY NOMINATIONS 2022: Outstanding Lead Actress In A Limited Or Anthology Series Or Movie, EMMY NOMINATIONS 2022: Outstanding Lead Actor In A Limited Or Anthology Series Or Movie. The Staphylococcus aureus ferments mannitol and turns the medium yellow. What bacteria Cannot Grow on mannitol salt agar? Which of the following organisms is gram-negative? Staphylococcus aureus produce yellow colonies with yellow zones, whereas other Staphylococci produce small pink or red colonies with no colour change to the medium. Other uncategorized cookies are those that are being analyzed and have not been classified into a category as yet. Prolonged incubation ( 48 hours) may also allow growth of Micrococcus, Bacillus, and some species of Serratia. c. Add water until run-off is clear. Staphylococcus can grow well on mannitol salt agar because it can tolerate high saline. The high salt concentration (7.5%) is the selective ingredient. 7.5% NaCl and only bacteria that can survive the high osmotic pressure due to the sodium chloride will be able to grow on this plate. Sorbitol and pH indicator: Gram negative enteric bacilli will grow; E. coli 0157:H7 does not ferment sorbitol and colonies are colorless: Mannitol Salt Agar (MSA. Susceptible, resistant, or intermediate Necessary cookies are absolutely essential for the website to function properly. The cookies is used to store the user consent for the cookies in the category "Necessary". c. The decolorizing agent dissolves the outer membrane. If you FORGOT to do the decolorizing step, what colors would the Gram-positive and Gram-negative cells be when viewed at the end of the procedure? Using mannitol salt agar helps demonstrate the ability of bacteri a to grow in a 7.5% salt . a. Magnification d. Nucleoid Table 1: composition of HiChrome Bacillus Agar Medium Composition Hicrome bacillus agar medium Ingredients Gms/litre Peptic digest of animal tissue 10.000 Meat extract 1.000 D-mannitol 10.000 Sodium chloride 10.000 Chromogenic mixture 3.200 Phenol red 0.025 Agar 15.000 Final pH(at 25oC) 7.1 0.2 Identification of Isolates: b. Safranin Bacillus , and some species of Serratia . Old Gram-positive cells will be decolorized. It is also required to detect and differentiate haemolytic bacteria, especially Streptococcus species. b. Sterilize by autoclaving at 15 lbs. But opting out of some of these cookies may affect your browsing experience. . Staphylococcus species are halotolerant, whereas Streptococcus species and many other organisms are inhibited by high concentrations of NaCl. Most pathogenic staphylococci, such as Staphylococcus aureus, will ferment mannitol. a. Streptobacilli The decolorizing agent dissolves the outer membrane. These cookies will be stored in your browser only with your consent. Beside above, does Bacillus subtilis Grow on mannitol salt agar? A dog loses a lot of heat through panting. Bacillus subtilis is grown on nutrient agar or in nutrient broth. B. cereus colonies are usually lecithinase-positive and mannitol-negative on MYP agar. Enterococcus faecalis does not go on the synthetic medium but does grow on the tryptic soy broth and the SF broth. If a particular specimen contains Staphylococcus aureus, you can expect that it can easily ferment mannitol. What is the purpose of using Gram's iodine during the Gram staining procedure? Would Bacillus cereus Grow on mannitol agar? Blood agar has the differential property due to its hemolysis property. MANNITOL SALT AGAR - For in vitro use only - Catalogue No. IV. Mannitol Salt is a selective bacterial growth medium because it has a very high concentration of NaCl (7.5%). This cookie is set by GDPR Cookie Consent plugin. 2022 - 2023 Times Mojo - All Rights Reserved a. Gram-positive cocci However, you may visit "Cookie Settings" to provide a controlled consent. What would be the likely consequences of removing the NaCl from MSA? Bacillus subtilis is not able to ferment mannitol and yet the Mannitol test yielded a positive result. Does Salmonella grow on mannitol salt agar? b. MacConkey agar The cookie is used to store the user consent for the cookies in the category "Other. c. catalase . rabbit plasma These data reveal that we now have rapid, cost effective ways to identify enterococci to the species, and not just genus, level and have significance for patient treatment in hospitals. It contains a high concentration (about 7.510%) of salt (NaCl) which is inhibitory to most bacteria making MSA selective against most Gram-negative and selective for some Gram-positive bacteria (Staphylococcus, Enterococcus and Micrococcaceae) that tolerate high salt concentrations. Mannitol Salt is a selective bacterial growth medium because it has a very high concentration of NaCl (7.5%). Bacillus species are rod-shaped, endospore-forming aerobic or facultatively anaerobic, Gram-positive bacteria; in some species cultures may turn Gram-negative with age. E coli is a gram-negative bacillus that grows well on commonly used media. b. What bacteria can grow on EMB agar? a. Bacillus subtilis grow in the mesophilic temperature range. What is the purpose of mannitol salt agar? Advertisement Bacillus subtilis can grow in any medium containing nitrogen and carbon source with Nacl. b. What kind of bacteria can grow on mannitol salt agar? b. Gram-positive rods Out of these, the cookies that are categorized as necessary are stored on your browser as they are essential for the working of basic functionalities of the website. Which of the following observations would suggest that a plate was inoculated with a pure (axenic) culture? c. 520 nm d. isolate bacteria and develop pure cultures d. Illumination, Which of the following microbes cannot be directly seen using our light microscopes? How to Market Your Business with Webinars. Some gram-positive bacteria, such as enterococci, staphylococci, and yeast will grow on this medium and usually form pinpoint colonies. b. Phenol red inhibits the growth of gram-positive organisms. Advertisement cookies are used to provide visitors with relevant ads and marketing campaigns. B. cereus food poisoning may occur when foods are prepared . a. quantify the number of bacteria Resolving power b. True/False When it ferments D-mannitol, it produces an acid c. Cells are not distorted by the effects of chemicals Whats the best temperature to grow Bacillus cereus? Would Bacillus cereus Grow on mannitol agar? Mannitol Salt Agar (MSA) is used as a selective and differential medium for the isolation and identification of Staphylococcus aureus from clinical and non-clinical specimens. What does Bacillus subtilis look like on an agar plate? In this manner, does Bacillus cereus ferment mannitol? Cetrimide agar is a type of agar used for the selective isolation of the gram-negative bacterium, Pseudomonas aeruginosa. Bacillus cereus is a Gram-positive, rod-shaped, facultatively anaerobic, motile, beta-hemolytic, spore forming bacterium commonly found in soil and food. True/False d. Isolated colonies are all white in color and about the same size. Can Bacillus cereus grow on MacConkey Agar? d. Add Gram's iodine. Bacillus subtilis is not able to ferment mannitol and yet the Mannitol test yielded a positive result. These cookies ensure basic functionalities and security features of the website, anonymously. The cookies is used to store the user consent for the cookies in the category "Necessary". d. Gram's iodine is a killing agent, binds to crystal violet, and serves as a mordant. Can Bacillus species grow on Mannitol salt agar. c. T-Soy + 10% NaCl c. All would be purple. The decolorizing agent dehydrates the peptidoglycan. c. Mannitol salt agar Rabbit plasma b. contamination of the agar The application of the mordant What purpose does phenol red serve in MSA? The phenol is a pH level indicator. Isolation of bacteria is accomplished by growing ("culturing") them on the surface of solid nutrient media. 2 Is Bacillus megaterium mannitol positive or negative? The colony morphologies of B. subtilis are highly variable, within and between strains which may give the appearance of a mixed culture during growth on an artificial medium. c. white to cream colored colonies surrounded by yellow halos in the agar, In the antiseptics/disinfectants lab, which organism appeared to be the most resistant to the antiseptics and disinfectants? Over time, gram-negative cells lose their ability to retain the crystal violet stain after decolorization. What is the gram stain morphology of this picture? Why? It causes the cells to adhere to the slide. Can You ferment lactose on mannitol salt agar? What media supports only Gram Negative and inhibits Gram positive bacteria? This cookie is set by GDPR Cookie Consent plugin. Mannitol Salt Agar (MSA) is a selective and differential medium. They are used for a wide range of applications, including culture storage, enumeration of cells . a. a. Bacillus (subtilis or megaterium) True/False Can You ferment mannitol with Bacillus subtilis? B. subtilis can ferment glucose, sucrose, but not lactose. MSA contains a high concentration of salt (NaCl), and therefore, selects for the growth of microbes that can tolerate high salt concentrations. b. Clusters of gram-positive cocci They are general-purpose, nonselective media providing enough nutrients to allow for a wide variety of microorganisms to grow. Will Bacillus Grow on . They are fairly large bacteria, with dimensions ranging from 3 0.4 to 9 2 m. So Staph grow well in this media. Will Bacillus Grow on mannitol salt agar? What are the differential ingredients in Mannitol Salt Agar? The large colonies at the center of the plate are Bacillus cereus. 7.5% sodium chloride inhibits the growth of most Gram . Bacillus subtilis Bacillus subtilis is not able to ferment mannitol and yet the Mannitol test yielded a positive result. The species of staphylococci can grow and tolerate the salt concentration .